WebFeb 13, 2024 · flp/frt 系统就属于位点特异性重组系统的一种。 FLP是一个重组酶(flippase,Flp),类似于Cre,它识别的位点是FRT,当FRT在同一条DNA上且方向相 … WebFigure 3-20. Relative expression of FLAG-hFFA4-mVenus and FLAG-hFFA4-GFP2 in a Flp-InTM T-RExTM 293 cell line. Flp-InTM T-RExTM 293 double stable cells able to express inducibly FLAG-hFFA4-mVenus and constitutively FLAG-hFFA4-GFP2 were seeded into poly-Dlysine coated black 96-well plates. Cells were treated with varying concentrations …
Hek293t Flp In Thermo Fisher Bioz
WebOct 31, 2024 · HEK293细胞及其衍生株HEK293(human embryonic kidney 293),简称293细胞,来源于人胚胎肾细胞,由于在19号染色体整合了大约4.35 kb的腺病毒基因组片段,使其细胞周期以及细胞表型、核型等发生了 ... 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性(ecotropic)报告质粒和 ... WebAbstract. Each subtype of the muscarinic receptor family of G protein-coupled receptors is activated by similar concentrations of the neurotransmitter acetylcholine or closely rel how much is the blaze subscription
www.jbc.org
WebHuman. The Flp-In T-REx-293 cell line stably expresses the lacZ-Zeocin fusion gene and the Tet repressor, and is designed for use with the Flp-In T-REx System. The cell line expresses the Tet repressor from pcDNA6/TR and contains a single integrated Flp Recombination Target (FRT) site from pFRT/lacZeo as confirmed by Southern blot analysis. WebThe Flp-In ™ T‑REx -293 cell line is shipped on dry ice. Store in liquid nitrogen vapor-phase upon receipt. The Flp-In™ T‑REx™-293 cell line is supplied as one vial containing 1 × … WebFeb 20, 2024 · 在我们以前的工作中,我们在四环素诱导型CMV启动子(HEK-293 Flp-In T-REx-Thermo)的控制下,建立了表达C末端3xFLAG标记的外来体成分EXOSC10(RRP6)的同基因HEK-293细胞系费雪科学)。. 该系统允许我们以与内源WT蛋白质相当的水平表达标记的EXOSC10蛋白质,并且使用磁性 ... how do i get a heloc loan