Webbreactions (PCR) was assessed using the genomic DNA samples isolated. PCR reactions were carried out in a final volume of 25 µl containing 2.5 mM sTris-Hcl (pH 8.3), 50 mM KCl, 5.0 mM MgCl 2, 250 µM of each dNTP, 1.0 µM bprimer, 1 U of AmpliTaq® DNA polymerase, and 75 ng DNA template. The Thermocycler used was Webb用pCX-DG质粒为模版进行PCR，克隆出目的基因条带gfp，用1%琼脂凝胶进行电泳，电泳检测如图1A所示。用试剂盒回收PCR产物，进行入门克隆，挑取经壮观霉素抗性筛选的单克隆5 mL-LB培养基培养过夜，菌液PCR鉴定，3个单克隆均扩出820 bp的目的条带(图1B)。

农杆菌介导的油菜子叶瞬时表达_百度文库

Webbthe fact that Taq polymerase functions at temperatures 40–60o higher than E. coli Pol I1/Klenow (1–5). A number of laborato- ... Due to its use in PCR, Taq polym-erase is one of the most important biotechnological reagents in use in the world today. The enzyme has both polymerase ac-tivity and 5 nuclease activity and is a member of the Pol I btn classroom ep 1

PCR Master Mix - Sigma-Aldrich

Webbmutagenesis, the PCR amplifications for the 16 exons of the wild-type hMSH2 (8) gene were performed on the GeneAm PCR System 960p 0 (Perkin-Elmer) in a 50-µL volume con-taining 1×PCR Buffer II (10 mM Tris-HCl, pH 8.3, 50 mM KCl; Perkin-Elmer), 3 mM MgCl2(except for exon 1, which used 1.5 mM MgCl2), 0.2 mM of each dNTP, 10 pmol … WebbMost recent answer. 10th Mar, 2024. Ahmad Ali. Panjab University. In PCR, MgCl2 is an essential cofactor that enhances the activity of Taq DNA polymerase, which in turn … WebbAdd to Cart. Add to Helix. Magnesium Chloride Solution. 1.5ml. $ 49.00. Your price: Log in. Divalent cations, such as Mg 2+, are required as a cofactor for DNA polymerases used in PCR. Thus, MgCl 2 concentration affects productivity and fidelity of the DNA polymerase. MgCl 2 also has an effect on primer binding to the target DNA. btn classroom 35