Tacc3 oocyte

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August undefined, 2024

WebNov 1, 2002 · A protein spot cored from a silver-stained two dimensional (2D) gel of germinal vesicle stage immature mouse oocytes was identified as Transforming Acidic Coiled Coil containing protein (TACC3)... WebApr 1, 2009 · TACC3 has a well characterized function in microtubule dynamics, particularly during mitosis, based on mutational analysis (see above) and physical interactions with Aurora A and Aurora B kinases, CKAP5 (ch-TOG/XMAP215) and AKAP9 via the TACC domain (see Peset and Vernos, 2008 for review).

Tacc3 transforming, acidic coiled-coil containing protein 3

WebJun 28, 2024 · Abstract. Mammalian oocytes segregate chromosomes with a microtubule spindle that lacks centrosomes, but the mechanisms by which acentrosomal spindles are organized and function are largely unclear. In this study, we identify a conserved … WebFeb 25, 2024 · Oocyte treatments with taxol, nocodazole, or cold did not affect CLTC expression levels but led to disorders of its distribution. Functional impairment of CLTC by specific morpholino injections in GV-stage oocytes led to disruptions in spindle assembly and chromosomal alignment, accompanied by impaired first polar body (PB1) emissions. poison ivy #1 nakayama exclusive variant

21335 - Gene ResultTacc3 transforming, acidic coiled-coil

WebAug 5, 2024 · TACC3, a member of the TACC family, is a dominant protein in this domain, which is essential to promote the formation of a highly dynamic liquid-like meiotic spindle domain (LISD) in mammalian oocytes (Peset and Vernos 2008; Wang et al. 2024). WebOct 14, 2024 · The primary antibodies were listed as follows: MTX2 (1:100 in dilution, 11610-1-AP, Proteintech), GDF9 (1:100 in dilution, ab93892, Abcam), TACC3 (1:50 in dilution, ab134154, Abcam), EIF5A (1:50 in dilution, 11309-1-AP, Proteintech), ASPM (1:150 in dilution, 26223-1-AP, Proteintech). Webthat TACC3 is required for acentrosomal microtubule assembly (Fig. 1 C and D and Fig. S1 C and D). To understand why TACC3 is required for the acentrosomal microtubule aster assembly, we treated the mitotic HeLa cells with nocodazole or cold temperatures and observed that TACC3 stayed with the stable kinetochore-connectedﬁbers poison ivy 1992 다시보기

Transforming acidic coiled-coil protein-3: a novel marker for ...

Highly Potent TACC3 Inhibitor as a Novel Anticancer Drug Candidate

WebMar 1, 2024 · Spindle assembly defects mediated by centrin TACC3 and decreased acetylation, microﬁlament reorganization failure, and mitochondrial dysfunction were found to be the underlying mechanisms involved in the toxicity of cobalt chloride to oocytes. WebJun 2, 2024 · TACC3, a transforming acidic coiled-coil (TACC) family member, is frequently upregulated in a broad spectrum of cancers, including breast cancer. It plays critical roles in protecting microtubule stability and centrosome integrity that is often dysregulated in cancers; therefore, making TACC3 a highly attractive therapeutic target. bank mandiri magetanWebJul 26, 2024 · TACC3 regulates spindle organization during mitosis and also regulates centrosome-mediated microtubule nucleation by affecting γ-Tubulin ring complexes. In addition, it interacts with different proteins (such as ch-TOG, clathrin and Aurora-A) to … poison ivy adalah

"WebJul 13, 2015 · As a result of the embryonic lethality, the function of TACC3 during oocyte meiosis remains unclear. Compared to mitosis, little is known about the mechanisms that drive and control meiosis in oocytes. Here, we show that TACC3 is expressed around the … " - Tacc3 oocyte

Tacc3 oocyte

TACC3 co-localization with tubulin. TACC3 co-localizes

WebJul 15, 2024 · We speculated that the function of TPX2 beyond microtubule assembly is conserved among species and may also be related to AURKA and TACC3. Here, porcine oocytes were selected to reveal the spatiotemporal and function of TPX2 in microtubule … WebRecently, the human oocyte microtubule organizing center was found to be a driver for spindle assembly in human oocytes, and deficiencies in TACC3, the key component of this center, were shown to impair microtubule nucleation and meiotic spindle assembly, leading to nuclear maturation arrest .

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WebSep 3, 2013 · TACC3 facilitates kinetochore–microtubule capture, interkinetochore tension, and spindle assembly checkpoint. Micrographs are presented as maximum intensity projections of different z sections. ( A) … WebNov 18, 2024 · We found that mutations in TACC3 cause defects in spindle assembly by disrupting the structure of the huoMTOC, which leads to clinical oocyte maturation arrest. This suggests that the huoMTOC might be an important biomarker for evaluating the …

WebJul 13, 2015 · TACC3 mRNA was detected in bovine oocytes during meiosis using qRT-PCR, and while it was also expressed in cleavage stage embryos, its expression was down-regulated at the mor-ula and... WebJul 13, 2015 · TACC3 mRNA was detected in bovine oocytes during meiosis using qRT-PCR, and while it was also expressed in cleavage stage embryos, its expression was down-regulated at the morula and blastocyst stages. Immunofluorescence was used to …

WebOct 2, 2008 · TACC3 is an indirect target of TPX2 in the oocyte. A: Localization of P-TACC3 upon TPX2 depletion. Oocytes were triple labelled for P-TACC3 (red), microtubules (green) and nucleic acids (blue). P-TACC3 staining is absent in siTPX2-5h, siTPX2-5h co-injected … WebThe TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension ( PubMed: 21297582, PubMed: 23532825 ). May be involved in the control of cell growth and differentiation. May contribute to cancer ( PubMed: 14767476 ). By similarity 3 publications GO annotations Expand table Complete GO annotation on QuickGO Keywords

WebTransforming acidic coiled-coil (TACC) proteins are key players during mitosis via stabilization of the spindle. The roles of TACCs during meiosis are however less clear. We used bovine oocytes...

WebSep 26, 2024 · Tacc3 is a negative regulator of the Notch signaling pathway. in addition to spindle assembly, Tacc3 has critical roles in chromosome separation and cytokinesis, and is essential for the mitosis of sclerotome mesenchymal cells during axial formation in … poison item osrsWebJun 28, 2024 · ( A) Immunofluorescence images of mouse oocytes fixed at different times after nuclear envelope breakdown (NEBD). Gray, microtubules (α-tubulin); green, LISD (TACC3); magenta, aMTOCs (pericentrin). Insets are magnifications of regions outlined by … poison ivy 1992 filmWebMay 1, 2024 · During mammalian oocyte development, two contrasting patterns of polyadenylation have been identified. ... SLBP (Abnova H00007884-M01, 200 ng/mL), tubulin (Sigma T8203, 10 μg/mL) and TACC3 ... bank mandiri magelangWebAug 5, 2024 · Notably, IPZ treatment decreased TPX2 expression and abnormal subcellular localization. Furthermore, the expression levels of aurora kinase A (AURKA) and transforming acidic coiled-coil 3 (TACC3)... bank mandiri magangWebSep 19, 2005 · TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A–dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis. … bank mandiri makassarWebFeb 18, 2024 · 3.4 Oocyte and granulosa cell communications We used iTALK to characterize the L-R–mediated intercellular communication and list the top 100 L-R pairs in antral stage oocytes and GCs (Table S2 ). Figure 3A … bank mandiri lowongan kerjaWebTACC3; transforming acidic coiled-coil containing protein 3. Aliases: ERIC1, Tacc4, ERIC-1, maskin. Location: 4p16.3. Summary: This gene encodes a member of the transforming acidic colied-coil protein family. The encoded protein is a motor spindle protein that may … poison ivy 1992