WebApr 10, 2024 · The ZDHHC3 lentiviral short hairpin RNA (shRNA) vector was designed and purchased from Tsingke (Beijing, China) and was used to construct stable transfection Panc 02 pancreatic cell lines. The PD-L1 overexpressing in ZDHHC3-knockdown Panc 02 cell line was constructed by a recombinant lentivirus (pLV-PD-L1-puro) and performed following … WebSep 15, 2024 · In China, largemouth bass ulcerative disease was first reported in the Guangdong province in 2008 ... PCR products were purified (Tiangen, China) and sequenced (TsingKe, China), they were aligned online using BLAST …
Tsingke
Webgel recovery kit (Tsingke, China), ligated with the pClone007 vector (Tsingke, China), and transformed into competent Escherichia coli cells. After transformation, six positive clones were picked for sequencing (Tsingke, China). To obtain the promoter sequence, a PCR gene walking approach was performed by using a universal genomewalk™ WebBased in Beijing, China, and established in 2024, Tsingke Biotechnology Co., Ltd. is a biotechnology company that focuses on R&D of synthetic genomics and biosynthetic … greene county vs west lyon
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WebJun 22, 2024 · The circSLC8A1 probe (Tsingke, Wuhan, China) was incubated with streptavidin magnetic beads (Life Technologies, USA) at room temperature for 2 h to generate probe-coated beads. The cell lysates were incubated with … WebApr 13, 2024 · The labeled probes with biotin (TSINGKE Biological Technology, China) and labeled RNAs were incubated with A549 cell nuclear extracts; then, the RNA pull-down assay was performed using the Pierce Magnetic RNA Protein Pull-Down Kit (20164, Thermo Fisher Scientific, USA). The proteins were analyzed by western blotting. WebSep 16, 2024 · DNA sequencing was performed by Tsingke Biotech Co., Ltd (Hangzhou, China). All the plasmids used in this study were listed in Table 1 and the corresponding … fluffy slippers from the 70s